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Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses
Published on: November 3, 2014
The complete mitochondrial genome of wolf spider Pirata subpiraticus Boes.et str. (Araneae: Lycosidae)
Zheng-Liang Wang1, Chao Li1, Wen-Yuan Fang1
1a Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine , College of Life Sciences, China Jiliang University , Hangzhou , Zhejiang , People's Republic of China.
Abstract:
The complete mitochondrial genome of a wolf spider Pirata subpiraticus was determined. It is a circular molecule of 14,528 bp in length and contains 13 protein-coding genes, 2 ribosomal RNAs, 21 transfer RNAs (tRNA(Gly) is lost) and a control region. The A + T content of the overall base composition of majority strand (J-strand) is 75.6% (T: 42.0%; C: 8.6%; A:33.6%; G: 15.8%). Among protein-coding genes, one genes (COIII) start with TTG, four genes (ND1, ND2, ATP8 and Cytb) begin with ATT and other eight genes use ATA as initiation codon. ND2 and Cytb are terminated with TAG as stop codon, while all other genes end with TAA. Three isolated sequences repeat regions (212 bp) were found in the A+T-rich region (D-loop).
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