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Purification of a High Molecular Mass Protein in Streptococcus mutans
Published on: September 14, 2019
Short communication: Conservation of Streptococcus uberis adhesion molecule and the sua gene in strains of
Ying Yuan1, Oudessa Kerro Dego2, Xueyan Chen2
1Zoetis, Veterinary Medicine Research and Development (VMRD), Global Therapeutic Research, 333 Portage Street, Kalamazoo, MI 49007.
Abstract:
The objective was to identify and sequence the sua gene (GenBank no. DQ232760; http://www.ncbi.nlm.nih.gov/genbank/) and detect Streptococcus uberis adhesion molecule (SUAM) expression by Western blot using serum from naturally S. uberis-infected cows in strains of S. uberis isolated in milk from cows with mastitis from geographically diverse areas of the world. All strains evaluated yielded a 4.4-kb sua-containing PCR fragment that was subsequently sequenced. Deduced SUAM AA sequences from those S. uberis strains evaluated shared >97% identity. The pepSUAM sequence located at the N terminus of SUAM was >99% identical among strains of S. uberis. Streptococcus uberis adhesion molecule expression was detected in all strains of S. uberis tested. These results suggest that sua is ubiquitous among strains of S. uberis isolated from diverse geographic locations and that SUAM is immunogenic.
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