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Tomato Male sterile 1035 is essential for pollen development and meiosis in anthers
Hee-Jin Jeong1, Jin-Ho Kang1, Meiai Zhao2
1Department of Plant Science and Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea Plant Genomics and Breeding Institute, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro Gwank-gu, Seoul 151-921, Republic of Korea.
Abstract:
Male fertility in flowering plants depends on proper cellular differentiation in anthers. Meiosis and tapetum development are particularly important processes in pollen production. In this study, we showed that the tomato male sterile (ms10(35)) mutant of cultivated tomato (Solanum lycopersicum) exhibited dysfunctional meiosis and an abnormal tapetum during anther development, resulting in no pollen production. We demonstrated that Ms10(35) encodes a basic helix-loop-helix transcription factor that is specifically expressed in meiocyte and tapetal tissue from pre-meiotic to tetrad stages. Transgenic expression of the Ms10(35) gene from its native promoter complemented the male sterility of the ms10(35) mutant. In addition, RNA-sequencing-based transcriptome analysis revealed that Ms10(35) regulates 246 genes involved in anther development processes such as meiosis, tapetum development, cell-wall degradation, pollen wall formation, transport, and lipid metabolism. Our results indicate that Ms10(35) plays key roles in regulating both meiosis and programmed cell death of the tapetum during microsporogenesis.
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