Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From superficial scholarship to algorithmic slop in analytical chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2026

Same author

Band-stop microfluidics for high-purity, label-free enrichment of viable cancer cells from whole blood.

Lab on a chip·2026

Same author

MaGIC-OT: an AI-guided optical tweezers platform for autonomous single-cell isolation in microfluidic devices.

Lab on a chip·2026

Same author

Affordable, cleanroom-free millifluidic production of targeted lipid nanocarriers <i>via</i> additive manufacturing.

Lab on a chip·2026

Same author

Assembling Lipid Membrane Scaffolds on Microgel-Based Artificial Cells through Vesicle Fusion onto the Hydrogel Network.

ACS nano·2026

Same author

Microfluidic Production of Spatially Structured Biomimetic Microgels as Compartmentalized Artificial Cells.

Small science·2025

Search research articles

Related Experiment Video

Updated: Apr 23, 2026

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Addressable droplet microarrays for single cell protein analysis.

Ali Salehi-Reyhani¹, Edward Burgin, Oscar Ces

¹Proxomics, Institute of Chemical Biology, Imperial College London, London, SW7 2AZ UK. ali.salehi-reyhani@imperial.ac.uk.

|September 30, 2014

Summary

We developed oil-encapsulated addressable droplet microarrays for high-sensitivity analyses. This method allows for single-cell analysis of protein variations in cancer cells without complex microfluidic devices.

More Related Videos

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Related Experiment Videos

Last Updated: Apr 23, 2026

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Addressable droplet microarrays offer miniaturized, high-sensitivity analysis potential.
Current methods often require complex microfluidic device fabrication or small chambers.

Purpose of the Study:

To report a practical method for producing oil-encapsulated addressable droplet microarrays.
To demonstrate the utility of these microarrays for single-cell analyses.

Main Methods:

Development of a method for creating oil-encapsulated addressable droplet microarrays.
Application of the microarrays for single-cell analysis of p53 protein copy number variation.

Main Results:

Successful production of functional oil-encapsulated addressable droplet microarrays.
Demonstrated utility in analyzing single human cancer cell line for p53 protein copy number variations.

Conclusions:

Oil-encapsulated addressable droplet microarrays provide a practical approach for miniaturized, high-sensitivity analyses.
This technology facilitates single-cell studies, such as determining protein copy number variations in cancer cells.