Spotted fever group rickettsiae in ixodid ticks in Oromia, Ethiopia

In Ethiopia, information on the transmission of human zoonotic pathogens through ixodid ticks remains scarce. To address the occurrence and molecular identity of spotted fever group rickettsiae using molecular tools, a total of 767 ixodid ticks belonging to thirteen different species were collected from domestic animals from September 2011 to March 2014. Rickettsia africae DNA was detected in 30.2% (16/53) Amblyommma variegatum, 28.6% (12/42) Am. gemma, 0.8% (1/119) Am. cohaerens, 18.2% (4/22) Amblyomma larvae, 6.7% (2/60) Amblyomma nymphs, 0.7% (1/139) Rhipicephalus (Boophilus) decoloratus and 25% (1/4) nymphs of Rh. (Bo.) decoloratus. A markedly low prevalence of R. africae was recorded in both Am. cohaerens and Rh. (Bo.) decoloratus (p<0.0001) compared with that in Am. variegatum and Am. gemma. The prevalence of R. africae was markedly low in the western districts (Gachi and Abdela) (p<0.0001); however, the prevalence of R. africae was relatively high in the central (Ada'a, Wolmara and Arsi) and eastern (Arero, Moyale and Yabelo) districts, where Am. variegatum and Am. gemma were predominantly associated with R. africae, respectively. R. aeschlimannii DNA was detected in 45.4% (5/11) Hyalomma marginatum rufipes and 2.2% (1/46) Hy. truncatum. Moreover, the first report of R. massiliae DNA in 1.9% (1/52) Rhipicephalus praetextatus ticks in Ethiopia is presented herein. Altogether, these results suggest that the transmission of spotted fever group rickettsiae through ixodid ticks is a potential risk for human health in different parts of Ethiopia. Clinicians in this country should consider these pathogens as a potential cause of febrile illness in patients.