Related Experiment Videos
ARIC hemostasis study--I. Development of a blood collection and processing system suitable for multicenter hemostatic
A C Papp1, H Hatzakis, A Bracey
1Vascular Disease Research Center, University of Texas Health Science, Houston.
Thrombosis and Haemostasis
|February 28, 1989
Summary
A new blood collection and processing system minimizes in vitro activation of hemostatic factors, ensuring reliable measurements for large-scale studies like the Atherosclerosis Risk In Communities (ARIC) study.
Area of Science:
- Clinical Chemistry
- Hematology
- Epidemiology
Background:
- Multicenter studies require standardized blood collection and processing to ensure data integrity.
- Accurate measurement of hemostatic factors is crucial for understanding atherosclerotic vascular disorders.
Purpose of the Study:
- To compare a novel blood collection and processing system with standard methods for multicenter studies.
- To validate a new system for reliable hemostatic factor measurement in epidemiological research.
Main Methods:
- Development of a new phlebotomy set, plasma filtration, and a mixture of anticoagulants/antiplatelet agents.
- Systematic evaluation of plasma beta TG, PF 4, FPA, and other hemostatic markers.
- Assessment of sample stability after storage and shipping.
Main Results:
- The new system yielded lower plasma beta TG, PF 4, and FPA values compared to reported standards.
- Reliable measurements were obtained for fibrinogen, factor VII, factor VIII, von Willebrand factor, antithrombin-III, protein C, tissue-type plasminogen activator, and serum thromboxane B2.
- Samples remained stable after -70°C storage and overnight shipping.
Conclusions:
- The developed blood collection and processing system effectively inhibits in vitro activation of hemostatic pathways.
- This system is suitable for multicenter clinical trials and epidemiological studies requiring consistent blood sample integrity.