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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Published on: July 4, 2011

Ionic Liquid-Based Fluorescein Colorimetric pH Nanosensors.

Susmita Das¹, Paul K S Magut¹, Sergio L de Rooy¹

¹Department of Chemistry, Louisiana State University, Baton Rouge, LA-70803, USA.

|September 30, 2014

Summary

Researchers developed a novel pH-sensitive ionic liquid nanosensor using fluorescein phosphonium salts. This colorimetric sensor shows distinct color changes in acidic conditions, indicating potential for disease detection.

Keywords:

J-aggregates colorimetric fluorescein pH phosphonium room temperature ionic liquids

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

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Published on: May 5, 2016

Area of Science:

Materials Science
Nanotechnology
Analytical Chemistry

Background:

pH is a critical biomarker for various physiological processes and diseases, including cancer.
Development of sensitive and selective pH nanosensors is crucial for early disease diagnosis and monitoring.
Ionic liquids offer unique properties for designing advanced nanomaterials.

Purpose of the Study:

To synthesize and characterize novel pH-sensitive, colorimetric ionic liquid nanosensors.
To investigate the optical and structural properties of these nanosensors in response to pH variations.
To evaluate the potential of these nanosensors for biomedical applications, particularly in pH-dependent disease detection.

Main Methods:

Synthesis of fluorescein salts with a trihexyltetradecylphosphonium cation ([TTP]+) in various stoichiometries ([TTP]2[FL] and [TTP][FL]).
Characterization of nanomaterials using Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) to assess size and morphology.
Spectroscopic analysis (absorption and fluorescence) and zeta potential measurements to understand pH-dependent optical properties and surface charge.

Main Results:

The synthesized nanomaterials exhibited distinct colorimetric responses in neutral versus acidic environments.
Significant expansion in nanodroplet/nanoparticle diameter was observed in acidic conditions, correlating with pH changes.
pH variations induced alterations in molecular orientations and stacking, affecting absorption and fluorescence spectra.

Conclusions:

The novel phosphonium-based fluorescein ionic liquid nanosensors demonstrate significant pH sensitivity and colorimetric output.
The observed changes in optical properties and size are directly linked to environmental pH.
These nanosensors hold promise as potential candidates for biomedical applications, especially for pH-based disease diagnostics like cancer detection.