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Updated: Apr 23, 2026

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
Published on: September 9, 2021
A versatile fluorescence turn-on assay for highly sensitive detection of tyrosine phosphatase activity
Chenghui Liu1, Fangfang Wang, Yucong Wang
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
Abstract:
A simple but robust fluorescence turn-on assay is developed for highly sensitive detection of PTP1B activity by using calcein as the signaling element.
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