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Subcellular localization of p29, a putative movement protein of pepper ringspot virus

Kelly B Rodrigues1, Anelise F Orílio, Rosana Blawid

  • 1Department of Cellular Biology, Post-graduation course of Molecular Biology, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil.

Archives of Virology
|October 1, 2014
PubMed

Insights

Pepper ringspot virus (PepRSV) movement protein (p29) localizes near cell walls. This suggests p29 facilitates PepRSV cell-to-cell movement, similar to other tobraviruses.

Area of Science:

  • Plant Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Pepper ringspot virus (PepRSV) is a Tobravirus with a positive-sense RNA genome.
  • The p29 protein, encoded by PepRSV RNA 1, is hypothesized to be the movement protein (MP).

Purpose of the Study:

  • To investigate the intracellular localization of the PepRSV p29 protein.
  • To determine if p29 functions as the movement protein in PepRSV.

Main Methods:

  • Transient expression of PepRSV p29 fused to green fluorescent protein (GFP).
  • Confocal microscopy to analyze protein localization.
  • Co-localization studies with a known movement protein (eCFP-tagged tobacco mosaic virus 30K MP).

Main Results:

  • PepRSV p29-GFP fusion protein exhibited punctate localization near the cell wall.
  • Partial co-localization was observed between PepRSV p29 and the tobacco mosaic virus 30K MP.

Conclusions:

  • The intracellular distribution of p29 suggests it associates with plasmodesmata.
  • These findings strongly indicate that p29 is the movement protein for Pepper ringspot virus.

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