Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural and functional studies on rabbit liver glycogenin.

C Smythe1, C Villar-Palasi, P Cohen

  • 1Department of Biochemistry, University of Dundee, Scotland.

European Journal of Biochemistry
|July 15, 1989
PubMed
Summary

Rabbit liver contains glycogenin, a protein essential for glycogen synthesis. This liver glycogenin is structurally and functionally identical to muscle glycogenin, indicating a shared biogenesis mechanism across tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electronic reporting of rare endocrine conditions within a clinical network: results from the EuRRECa project.

Endocrine connections·2023
Same author

Supporting international networks through platforms for standardised data collection-the European Registries for Rare Endocrine Conditions (EuRRECa) model.

Endocrine·2021
Same author

Are multiple checkpoint mediators involved in a checkpoint linking histone gene expression with DNA replication?

Biochemical Society transactions·2007
Same author

Activation of mammalian Chk1 during DNA replication arrest: a role for Chk1 in the intra-S phase checkpoint monitoring replication origin firing.

The Journal of cell biology·2001
Same author

INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow.

Current biology : CB·2000
Same author

Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs.

The EMBO journal·2000

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Metabolic Regulation

Background:

  • Glycogenin is the essential protein primer for glycogen synthesis in muscle.
  • The presence and role of glycogenin in the liver remain less understood compared to muscle.

Purpose of the Study:

  • To isolate and characterize glycogenin from rabbit liver.
  • To compare liver glycogenin with muscle glycogenin for structural and functional similarities.
  • To elucidate the mechanism of glycogen biogenesis in the liver.

Main Methods:

  • Isolation of glycogenin from rabbit liver glycogen.
  • Quantitative analysis of glycogenin content in liver glycogen.
  • Structural analysis, including amino acid sequencing around the modified tyrosine residue.

Related Experiment Videos

  • Partial purification and functional assay of liver glycogenin's autoglucosylation activity.
  • Comparison with purified muscle glycogenin.

    • Main Results:

    • Glycogenin was isolated from rabbit liver glycogen, comprising 0.0025% of the total mass.
    • Liver glycogenin content was found to be 200-fold lower than that in muscle glycogen.
    • Structural analysis confirmed liver glycogenin is identical to muscle glycogenin.
    • Partially purified liver glycogenin catalyzed an Mn2+- and UDP-Glc-dependent autoglucosylation reaction, similar to muscle glycogenin.
    • Approximately one glycogenin molecule was found per liver glycogen alpha particle.

    Conclusions:

    • Hepatic and muscle glycogenins are identical proteins.
    • Liver and skeletal muscle share a common mechanism for glycogen biogenesis.
    • The low abundance of liver glycogenin suggests tissue-specific regulation of glycogen synthesis initiation.