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Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Translation

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...

Translation

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...

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Related Experiment Video

Updated: Apr 23, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

U2AF1 mutations alter splice site recognition in hematological malignancies.

Janine O Ilagan¹, Aravind Ramakrishnan², Brian Hayes³

¹Computational Biology Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA; Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA;

Genome Research

|October 1, 2014

Summary

Mutations in U2AF1, a key RNA splicing factor, alter gene splicing in leukemias. These changes affect critical cellular pathways, offering insights into cancer development and U2AF1 function.

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

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Published on: October 9, 2014

Area of Science:

Molecular Biology
Genetics
Cancer Research

Background:

Whole-exome sequencing reveals frequent mutations in RNA splicing genes in hematological malignancies.
U2AF1 is a crucial factor in recognizing 3' splice sites during RNA splicing.

Purpose of the Study:

To investigate how U2AF1 mutations impact its function in RNA splicing.
To understand the downstream effects of U2AF1 mutations on gene expression and cellular pathways.

Main Methods:

Analysis of U2AF1 mutations in patient samples and cell cultures.
In vitro splicing assays and computational modeling.
Differential splicing analysis of hundreds of genes.

Main Results:

U2AF1 mutations alter preferred 3' splice site motifs, leading to differential splicing of numerous genes.
Affected pathways include DNA methylation, X chromosome inactivation, DNA damage response, and apoptosis.
Mutations in different U2AF1 zinc finger domains result in distinct splicing alterations.

Conclusions:

U2AF1 mutations contribute to cancer pathogenesis through quantitative changes in RNA splicing affecting diverse cellular processes.
The findings provide insights into the normal function of U2AF1's zinc finger domains in RNA splicing.