Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

15.9K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
15.9K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

9.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
9.1K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.3K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

11.0K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Waveguide-integrated colour centres in silicon carbide with broadband photonic crystal reflectors for efficient readout.

Npj nanophotonics·2026
Same author

Readout of a solid state spin ensemble at the projection noise limit.

Nature communications·2026
Same author

Automated measurement of intraperitoneal pressure versus patient fill-volume.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis·2026
Same author

Teclistamab Treatment Followed by Heart Transplantation for Advanced Immunoglobulin Light Chain Amyloid Cardiomyopathy.

JACC. Case reports·2026
Same author

Hierarchical maximum likelihood estimation for time-resolved NMR data.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same author

Open Circuit Voltage Enhancement and Defect Suppression of Wide-Bandgap CsPbIBr<sub>2</sub> Perovskite Solar Cells by Phenylhydrazinium Chloride Incorporation.

ACS omega·2026
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Apr 23, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.5K

Single-spin stochastic optical reconstruction microscopy.

Matthias Pfender1, Nabeel Aslam1, Gerald Waldherr1

  • 1Third Institute of Physics, Stuttgart Research Center of Photonic Engineering and Center for Integrated Quantum Science, University of Stuttgart, 70550 Stuttgart, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 2014
PubMed
Summary
This summary is machine-generated.

This study precisely locates single quantum emitters using advanced microscopy and spin resonance. The technique enables nanoscale sensing and improved spatial discrimination of quantum systems like nitrogen vacancy centers.

Keywords:
diamond defect centernitrogen-vacancy centersingle-spin detectionsuperresolution microscopy

More Related Videos

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

3.7K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K

Related Experiment Videos

Last Updated: Apr 23, 2026

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

10.5K
Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions
09:36

Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles in Three Dimensions

Published on: August 26, 2021

3.7K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.8K

Area of Science:

  • Quantum Optics
  • Nanotechnology
  • Spectroscopy

Background:

  • Single-quantum emitters are crucial for quantum technologies.
  • Precise spatial localization and characterization of these emitters remain challenging.
  • Nitrogen vacancy (NV) centers in diamond are promising quantum systems.

Purpose of the Study:

  • To demonstrate precision addressing of single-quantum emitters.
  • To combine optical microscopy and spin resonance for enhanced spatial resolution.
  • To develop a technique for nanoscale sensing applications.

Main Methods:

  • Utilized nitrogen vacancy (NV) color centers in diamond.
  • Developed a stochastic optical reconstruction microscopy (STORM) technique tailored for NV centers.
  • Performed simultaneous sub-diffraction-limit imaging and optically detected magnetic resonance (ODMR) measurements.

Main Results:

  • Achieved nanometer-scale resolution for assigning spin resonance spectra to individual NV center locations.
  • Successfully resolved formerly indistinguishable emitters based on their spectra.
  • Demonstrated sub-diffraction-limit sensing of magnetic and electric fields, including nuclear spins, with nanometer precision.

Conclusions:

  • The combined STORM and ODMR technique enables precise localization and characterization of quantum emitters.
  • This method significantly improves spatial discrimination and sensing capabilities.
  • Future applications include parallel quantum sensing for nanoscale imaging of quantum correlations.