Related Experiment Video
Updated: Apr 23, 2026

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
Published on: December 18, 2020
Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative
Zhenzhen Deng1, Mingliang Ye, Yangyang Bian
1Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China. mingliang@dicp.ac.cn hanfazou@dicp.ac.cn.
Abstract:
A simple, cost-effective and high throughput method was developed for multiplexed kinase activity assay based on the multiplex isotope labeling of designed substrate peptides. This strategy was successfully applied to monitor the time-dependent consumption of substrates and generation of products in the single and multiple substrate systems.
Related Concept Videos
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry

