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Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
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Combining experimental evolution with next-generation sequencing: a powerful tool to study adaptation from standing
C Schlötterer1, R Kofler1, E Versace2
1Institut für Populationsgenetik, Vetmeduni Vienna, Vienna, Austria.
Heredity
|October 2, 2014
Summary
Evolve and resequence (E&R) uses whole genome sequencing to find genetic changes during experimental evolution. This powerful method can identify genes and DNA variants linked to specific traits.
Area of Science:
- Evolutionary biology
- Genomics
- Molecular biology
Background:
- Experimental evolution is a key method for studying adaptation.
- Understanding the genetic basis of adaptation requires advanced genomic tools.
Purpose of the Study:
- To review the Evolve and Resequence (E&R) method for studying genomic responses to selection.
- To assess the power and limitations of E&R for identifying genetic variants.
- To propose strategies for optimizing E&R in evolutionary research.
Main Methods:
- Utilizes whole genome sequencing of pooled individuals (Pool-Seq).
- Applies controlled and replicable experimental evolution settings.
- Analyzes genomic data to detect selected variants.
Main Results:
- E&R is a powerful approach capable of identifying causative genes and single-nucleotide polymorphisms.
- Experimental design and trait complexity can lead to false positive candidate variants.
- Strategies are suggested to enhance the accuracy of variant detection.
Conclusions:
- Evolve and Resequence (E&R) is a valuable tool for linking genotype to phenotype.
- Optimized experimental and analytical approaches are crucial for maximizing E&R's utility.
- E&R can address a wide range of questions in evolutionary biology.
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