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Related Experiment Videos

Persistent immunogenicity of rat thymic epithelium.

H M Georgiou1, D Bellgrau

  • 1Barbara Davis Center for Childhood Diabetes, Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.

Transplantation
|August 1, 1989
PubMed
Summary

2-deoxyguanosine (2dGua) treatment enhances thymus tissue survival in mice but surprisingly causes rejection in rats. Rat thymus tissue, even after 2dGua treatment and "parking," is rejected, suggesting innate immunogenicity of thymic epithelium.

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Area of Science:

  • Immunology
  • Transplantation Biology
  • Developmental Biology

Background:

  • Thymus tissue transplantation is limited by immune rejection.
  • 2-deoxyguanosine (2dGua) treatment selectively depletes bone marrow-derived cells, reducing immunogenicity.
  • Mouse thymus grafts treated with 2dGua show enhanced survival in allogeneic recipients.

Purpose of the Study:

  • To investigate the efficacy of 2dGua treatment in reducing immunogenicity of rat thymus tissue.
  • To determine if residual marrow-derived cells are responsible for rejection of treated rat thymus grafts.
  • To assess the innate immunogenicity of rat thymic epithelium.

Main Methods:

  • Neonatal DA strain rat thymus tissue was cultured with 4 mM 2dGua.
  • Treated thymus tissue was transplanted under the kidney capsule of allogeneic PVG recipients.

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  • Treated thymus tissue was "parked" in T cell-depleted PVG rats before transplantation into normal recipients.

    • Main Results:

    • Contrary to mouse models, 2dGua treatment of rat thymus tissue resulted in acute rejection.
    • Rat thymus tissue, even after 2dGua treatment and prolonged "parking" in T cell-depleted recipients, was still acutely rejected upon re-transplantation.
    • Rejection occurred despite doses of 2dGua that effectively destroyed rat thymocytes in vitro.

    Conclusions:

    • Rat thymic epithelium, even when devoid of marrow-derived cells, is inherently immunogenic.
    • 2dGua treatment is not sufficient to prevent rejection of rat thymus grafts in allogeneic recipients.
    • The immunogenicity of rat thymus tissue may stem from the epithelial component itself.