Related Experiment Video
Updated: Apr 23, 2026
Multimodal Optical Imaging Platform for Studying Cellular Metabolism
Published on: June 6, 2025
Profiling metabolic states with genetically encoded fluorescent biosensors for NADH
1Synthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
NADH and its oxidized form, NAD(+), play central roles in energy metabolism and are ideal indicators of cellular metabolic states. In this review, we will introduce recent progress made in the developing of a series of genetically encoded NADH sensors, which offer the potential to fill the gap in currently used techniques of endogenous NAD(P)H fluorescence imaging. These sensors are bright, specific and organelles targetable, allowing real-time tracking and quantification of intracellular NADH levels in different subcellular compartments. The individual strengths and weaknesses of these sensors when applied to the study of metabolic states profiling will be also discussed.
