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Inositol trisphosphate induces calcium release from Neurospora crassa vacuoles
G Cornelius1, G Gebauer, D Techel
1Institute for Genetics, Saarland University, Saarbrücken, Fed. Rep. Germany.
Biochemical and Biophysical Research Communications
|July 31, 1989
Abstract:
Inositol 1,4,5-trisphosphate is known to release calcium ions from intracellular stores thought to be parts of endoplasmic reticulum in animal cells. In Neurospora crassa, however, inositol 1,4,5-trisphosphate acts on vacuoles stimulating a calcium efflux with a Km of 5.28 microM. The calcium release is inhibited effectively by dantrolene. These results were obtained by applying two independent methods, measuring calcium binding to fura-2 and loading vacuoles with 45Ca.