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Sample preparation methods for LC-MS-based global aqueous metabolite profiling.

Antoni Beltran1, Sara Samino, Oscar Yanes

  • 1Metabolomics Platform, Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM), Barcelona, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|October 2, 2014
PubMed
Summary
This summary is machine-generated.

This study presents two metabolite extraction protocols for untargeted metabolomic analyses. These methods optimize the detection of polar metabolites using liquid chromatography-mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR).

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Area of Science:

  • Metabolomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Metabolite extraction is crucial for untargeted metabolomic studies.
  • The chosen extraction method dictates detectable metabolites and analytical platform suitability.

Purpose of the Study:

  • To describe two distinct protocols for polar metabolite extraction from biological samples.
  • To facilitate the detection of reduced species via LC/MS.
  • To enable simultaneous analysis using both NMR and LC/MS.

Main Methods:

  • Protocol 1: Optimized for reduced polar metabolite detection by LC/MS.
  • Protocol 2: Designed for compatibility with both NMR and LC/MS analysis.

Main Results:

  • Successful extraction of polar metabolites.
  • Demonstrated utility of protocols for specific analytical techniques.
  • Enabled comprehensive polar metabolite profiling.

Conclusions:

  • The described protocols enhance the scope and efficiency of polar metabolite detection.
  • These methods support versatile metabolomic analyses using LC/MS and NMR.
  • Optimized extraction is key for robust untargeted metabolomic studies.