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Shotgun Lipidomics of Rodent Tissues
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Multidimensional mass spectrometry-based shotgun lipidomics.

Miao Wang1, Xianlin Han

  • 1Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute, 6400 Sanger Road, Orlando, FL, 32827, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 2, 2014
PubMed
Summary

Multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) provides efficient and sensitive analysis of cellular lipidomes. This study details a protocol for lipid sample preparation and mass spectrometry analysis, aiding researchers in managing this technology.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) is a key technology for lipid analysis.
  • It offers high efficiency, sensitivity, reproducibility, and broad coverage of lipid species.
  • MDMS-SL is crucial for studying lipid alterations in diseases, genetic modifications, drug treatments, and aging.

Purpose of the Study:

  • To present a detailed protocol for routine lipidomic analysis using MDMS-SL.
  • To describe lipid sample preparation from diverse biological materials.
  • To facilitate researchers' understanding and application of MDMS-SL for cellular lipidome analysis.

Main Methods:

  • Detailed protocol for lipid sample preparation from biological samples.
  • Mass spectrometry-based shotgun lipidomics (MDMS-SL) analysis.
  • Discussion of underlying principles of the technology.

Main Results:

  • A comprehensive protocol for analyzing numerous lipid classes and subclasses is presented.
  • Emphasis on the critical role of lipid sample preparation.
  • The protocol is adaptable for analyzing additional lipid classes.

Conclusions:

  • The presented protocol aids researchers in understanding and managing MDMS-SL.
  • It supports routine analysis of cellular lipidomes.
  • The methodology is valuable for diverse lipidomics research applications.