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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Related Experiment Video

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Author Spotlight: Investigating Fungal Pathogenicity Mechanisms in Maize
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In Planta Microsphere-Based Lateral Flow Leaf Biosensor in Maize.

Jessica T Wen1, Carlos Castro2, Hideaki Tsutsui3

  • 1Department of Mechanical Engineering, University of California, Riverside, CA, USA Department of Bioengineering, University of California, Riverside, CA, USA.

Journal of Laboratory Automation
|October 2, 2014
PubMed
Summary
This summary is machine-generated.

A novel biosensor technology detects biotic stresses directly within maize leaves. This low-cost, in planta system offers a user-friendly field detection method for protecting crops in developing nations.

Keywords:
biosensorlateral flow assaymaizemicrosphereplant biotechnology

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Area of Science:

  • Agricultural Science
  • Biotechnology
  • Plant Pathology

Background:

  • Biotic stress detection is crucial for maize crop protection in developing countries.
  • Smallholder farms face limitations in accessing advanced agricultural inputs.
  • Current detection methods are often costly and inaccessible for resource-limited settings.

Purpose of the Study:

  • To develop a low-cost, rapid, and field-deployable detection technology for biotic stresses in maize.
  • To integrate a lateral flow detection system directly within the plant (in planta).
  • To optimize and validate the technology using a mock biomarker in live maize plants.

Main Methods:

  • Development of a lateral flow biosensor using microspheres conjugated with analyte-specific antibodies.
  • Non-invasive injection of antibody-conjugated microspheres into maize leaves.
  • Optimization of microsphere size for efficient infiltration and immobilization within leaf tissues.
  • Demonstration of detection using a fluorescent mock biomarker (fluorescein) in live maize plants.

Main Results:

  • Successful optimization of microsphere size for in planta integration.
  • Demonstrated detection of a fluorescent mock biomarker within a live maize plant.
  • The developed system functions as an in planta lateral flow biosensor.

Conclusions:

  • The novel in planta lateral flow biosensor represents a first-of-its-kind technology.
  • This biosensor offers a promising low-cost and user-friendly solution for field-based biotic stress detection in maize.
  • The technology has the potential to significantly aid crop protection strategies for smallholder farmers.