Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil
Gustavo Freire Figueira1, Victor Henrique Silva de Oliveira2, Alessandra Taroda3
1School of Veterinary Medicine, Universidade Norte do Paraná, Arapongas, PR, Brazil.
Abstract:
This study investigated the occurrence of Eurytrema spp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.
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