Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.0K
CRISPR01:59

CRISPR

46.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.2K
CRISPR01:59

CRISPR

13.7K
13.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

14.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genome-wide association and multi-omics functional screens reveal the genetic architecture of foveal development.

medRxiv : the preprint server for health sciences·2026
Same author

Leukemia risk factor ARID5B coordinates HDAC-mediated transcriptional repression.

Nucleic acids research·2026
Same author

Distal enhancer-insulator module of GDF6 is essential for cochlear formation.

JCI insight·2026
Same author

Heterozygous loss-of-function alleles associate the conserved 3'-5' exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5-fluorouracil.

Molecular oncology·2026
Same author

Hepatocyte-Specific Deletion of Betaine-Homocysteine Methyltransferase Disrupts Methionine Metabolism and Promotes the Spontaneous Development of Hepatic Steatosis.

Biomolecules·2026
Same author

Disruption of the SAGA CORE triggers collateral degradation of KAT2A.

Nature communications·2026

Related Experiment Video

Updated: Apr 23, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K

Mouse Genome Editing Using the CRISPR/Cas System.

Donald W Harms1, Rolen M Quadros, Davide Seruggia

  • 1Mouse Genome Engineering Core Facility, Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, Nebraska; These authors contributed equally to this work.

Current Protocols in Human Genetics
|October 2, 2014
PubMed
Summary
This summary is machine-generated.

The CRISPR/Cas system enables rapid, multiplexed genome editing in mice. Protocols are provided for creating knockout and knock-in mouse models for biomedical research.

Keywords:
CRISPR/Cas9gene editingmutant mousepronuclear and cytoplasmic injectionsgRNA

More Related Videos

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.9K

Related Experiment Videos

Last Updated: Apr 23, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.5K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.2K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.9K

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Biomedical Research

Background:

  • Laboratory mice are crucial model organisms in biomedical research, particularly for human genetics.
  • Existing techniques for genetic mutation creation are often time-consuming.
  • The CRISPR/Cas system offers a novel approach to genome editing.

Purpose of the Study:

  • To provide detailed protocols for utilizing the CRISPR/Cas system in mouse models.
  • To facilitate the rapid creation of genetically modified mice.
  • To enable the generation of both knockout and knock-in mouse models.

Main Methods:

  • Preparation of CRISPR/Cas reagents.
  • Microinjection of reagents into one-cell mouse embryos.
  • Generation of genetically engineered mouse models.

Main Results:

  • Demonstrated the efficiency of the CRISPR/Cas system for mouse genome editing.
  • Successfully created multiple mutations in a single experimental procedure.
  • Established protocols for generating knockout and knock-in mouse models.

Conclusions:

  • The CRISPR/Cas system significantly accelerates the generation of genetically modified mice.
  • This technology enhances the utility of mice as model organisms in biomedical research.
  • The provided protocols streamline the creation of custom mouse models for diverse research applications.