Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

12.8K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative single-cell analysis of PML-RARĪ± oncogene-induced DNA damage along cell cycle progression.

Methods (San Diego, Calif.)Ā·2026
Same author

The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics.

PloS oneĀ·2026
Same author

Automated Intelligent Microscopy for Phenotype Identification, Spatial Localization, and Retargeting of Cells.

Methods in molecular biology (Clifton, N.J.)Ā·2026
Same author

Endocytic Control of Cell-Autonomous and Non-Cell-Autonomous Functions of p53.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)Ā·2026
Same author

Location of oncogene-induced DNA damage sites revealed by quantitative analysis of a DNA counterstain.

European biophysics journal : EBJĀ·2025
Same author

Enforcement of stem-cell dormancy by nucleophosmin mutation is a critical determinant of unrestricted self-renewal during myeloid leukemogenesis.

HaematologicaĀ·2025

Related Experiment Video

Updated: Apr 23, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

14.1K

High-resolution cytometry for high-content cell cycle analysis.

Laura Furia1, Piergiuseppe Pelicci, Mario Faretta

  • 1Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.

Current Protocols in Cytometry
|October 2, 2014
PubMed
Summary
This summary is machine-generated.

Automated microscopy offers an intracellular view, overcoming flow cytometry limitations. This method enables high-content cell cycle analysis with detailed cellular parameter evaluation.

Keywords:
S phaseautomated microscopycell cyclehigh resolutionhigh-content analysisimage cytometrywide-field microscopy

More Related Videos

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

19.1K
Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research
05:22

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

Published on: June 21, 2024

1.0K

Related Experiment Videos

Last Updated: Apr 23, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

14.1K
Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

19.1K
Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research
05:22

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

Published on: June 21, 2024

1.0K

Area of Science:

  • Cellular Biology
  • Microscopy Techniques
  • Quantitative Cytometry

Background:

  • Flow cytometry (FCM) lacks intracellular visualization capabilities, limiting comprehensive cellular analysis.
  • Automated microscopy and image analysis offer high-resolution, high-content data acquisition.
  • Translating FCM assays to high-content imaging remains an underexplored area.

Purpose of the Study:

  • To implement an automated microscopy protocol for cell cycle evaluation.
  • To bridge the gap between FCM assays and high-content image screening.
  • To enable simultaneous analysis of multiple cellular parameters.

Main Methods:

  • Development of an acquisition and analysis protocol for automated microscopy.
  • Focus on hardware features to optimize image data quality.
  • Detailed description of acquisition and analysis procedures for reliable results.

Main Results:

  • Successful implementation of a protocol for high-content cell cycle analysis using automated microscopy.
  • Demonstration of simultaneous analysis of numerous cellular parameters.
  • Establishment of procedures for generating high-quality image data.

Conclusions:

  • Automated microscopy provides an effective intracellular view, overcoming FCM limitations.
  • The developed protocol facilitates high-content screening of cell cycle assays.
  • This approach enhances quantitative cellular analysis through high resolution and statistical power.