Computer-supported device for histomorphometric investigations on laser-surgery.

Area of Science:

• Biomedical Engineering
• Surgical Technology
• Histopathology

Background:

• Laser and electrosurgery are common surgical tools.
• Accurate assessment of tissue alterations is crucial for surgical evaluation.
• Existing methods for morphometric analysis can be time-consuming or lack precision.

Purpose of the Study:

• To develop a device for quantitative morphometric analysis of tissue alterations.
• To enable interactive evaluation of histological specimens from experimental surgery.
• To provide precise measurements independent of microscopic magnification.

Main Methods:

• Development of a device integrating a microscope, scanning stage, digitizing tablet, and PC software.
• Quantitative measurement of incision depth, coagulation zone width, and area.
• Data export in standard formats (ASCII, HPGL, DXF) for further analysis.
• Application demonstrated on CO2-laser incisions in porcine oral mucosa.

Main Results:

• The device allows for independent measurement of key morphometric parameters.
• Quantitative data on tissue alterations from experimental laser surgery were obtained.
• The system facilitates detailed analysis of surgical specimen characteristics.

Conclusions:

• The developed device provides a robust solution for quantitative morphometric analysis of surgical tissue alterations.
• This tool enhances the evaluation of laser and electrosurgery effects in experimental settings.
• The system's ability to export data supports further research and clinical application.