Research on protein thermal condensation detection based on phase modulation SPR imaging

Area of Science:

• Biophysics
• Analytical Chemistry
• Proteomics

Background:

• Biomolecular interaction analysis is crucial for understanding biological processes.
• Existing Surface Plasmon Resonance (SPR) imaging methods have limitations in real-time phase change detection.

Purpose of the Study:

• To introduce a novel SPR imaging method utilizing time domain phase modulation (TDPM) for enhanced biomolecular interaction detection.
• To establish and validate an experimental apparatus for TDPM-based SPR imaging.
• To assess the method's capability in real-time phase change acquisition and kinetic parameter calculation.

Main Methods:

• Development of an SPR imaging apparatus incorporating TDPM.
• Preparation of a lysozyme array chip for experimental validation.
• Application of the Stoilov algorithm to extract phase information and SPR curves.
• Detection of lysozyme thermal condensation to demonstrate biomolecular interaction analysis.

Main Results:

• Successful establishment of a TDPM-based SPR imaging experimental setup.
• Real-time detection and analysis of biomolecular interactions, specifically lysozyme thermal condensation.
• Accurate extraction of phase information, SPR curves, and kinetic parameters.
• Demonstration of sensitive phase change acquisition based on interference imaging.

Conclusions:

• The novel TDPM SPR imaging method provides sensitive, real-time detection of biomolecular interactions.
• The Stoilov algorithm effectively processes phase information for kinetic analysis.
• This technique shows significant potential as a tool for advancing proteomics research.