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Flow Cytometric Characterization of Murine B Cell Development
Published on: January 22, 2021
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B cell lymphogenesis in swine is located in the bone marrow
Marek Sinkora1, Jana Sinkorova2
1Laboratory of Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., 549 22 Novy Hradek, Czech Republic marek.sinkora@worldonline.cz.
Journal of Immunology (Baltimore, Md. : 1950)
|October 3, 2014
Summary
This study identifies the bone marrow (BM) as the primary site for B cell development in swine. Researchers mapped the sequential stages of B cell lymphogenesis throughout life in pigs.
Area of Science:
- Immunology
- Developmental Biology
- Swine Genetics
Background:
- The precise location and developmental pathway of B cells in swine have remained unclear.
- Understanding B cell lymphogenesis is crucial for swine immunology and disease resistance.
Purpose of the Study:
- To pinpoint the site of B cell lymphogenesis in swine.
- To elucidate the sequential stages and characteristics of B cell development in porcine bone marrow (BM).
Main Methods:
- Flow cytometry using markers like MHC class II (MHC-II), CD2, CD21, CD25, CD45RC, CD172a, SWC 7, and μHC.
- Analysis of rearrangement-specific products and transcripts.
- Cell cultivation studies.
- Colocalization experiments.
Main Results:
- Porcine BM contains seven distinct B cell subsets representing sequential developmental stages.
- A clear developmental pathway was confirmed through molecular and cultivation analyses.
- Earliest precursors can revert to myeloid lineage, while later stages cannot.
- B lymphogenesis occurs throughout life in adult pigs' BM.
Conclusions:
- The bone marrow is the definitive site for B cell lymphogenesis in swine.
- This study provides the first comprehensive map of B cell development in pigs.
- Porcine BM is fully functional for B cell development across all adult life stages.
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