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Amelioration of insulin resistance by Rk1 + Rg5 complex under endoplasmic reticulum stress conditions

Shree Priya Ponnuraj1, Fayeza Siraj1, Sera Kang1

  • 1Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Suwon, Korea.

Pharmacognosy Research
|October 3, 2014
PubMed
Abstract

Insights

The Rk1 +Rg5 complex from heat-processed ginseng improves glucose uptake and insulin sensitivity in cells experiencing endoplasmic reticulum stress. This ginsenoside treatment helps overcome stress-induced insulin resistance.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Diabetes mellitus is exacerbated by stress, particularly endoplasmic reticulum stress (ERS), which impairs insulin signaling.
  • Ginsenosides, derived from Panax ginseng, are recognized for their therapeutic properties, including anti-diabetic effects.
  • ERS negatively impacts insulin resistance, necessitating external pharmacological interventions.

Purpose of the Study:

  • To evaluate the efficacy of the Rk1 +Rg5 complex in mitigating insulin resistance in 3T3-L1 cells.
  • To investigate the effects of Rk1 +Rg5 under induced endoplasmic reticulum stress conditions.

Main Methods:

  • Utilized heat-processed ginseng extracts rich in Rk1 +Rg5.
  • Employed 3T3-L1 cells as an insulin-resistant model subjected to ERS.
  • Conducted MTT assays, glucose oxidase assays, and RT-PCR/real-time PCR for gene expression analysis.

Main Results:

  • Rk1 +Rg5 treatment significantly enhanced glucose uptake compared to tunicamycin (TM) control.
  • Gene expression analysis revealed Rk1 +Rg5 influenced IGF 2R, CHOP-10, and C/EBP genes at 50 μM.
  • Ginsenoside treatment at 50 μM effectively counteracted approximately 50% of the induced stress.

Conclusions:

  • The Rk1 +Rg5 complex demonstrates a protective effect against insulin resistance in 3T3-L1 cells under ERS.
  • This study highlights the potential of Rk1 +Rg5 as a therapeutic agent for stress-induced insulin resistance.
Keywords:
3T3-L1dexamethasonediabetesginsenosidesinsulininsulin resistance

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