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Updated: Apr 23, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts
Mizuho Ichinose1, Masato Uchida1, Mamoru Sugita1
1Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
Abstract:
The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW-subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P. patens. Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14-C2 site, whereas over-expression of PpPPR_45 increased the levels of RNA editing at both the rps14-C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites.
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