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Nuclear protein p68 is an RNA-dependent ATPase
The EMBO Journal
|June 1, 1989
Summary
Human nuclear antigen p68, a helicase superfamily member, exhibits RNA-dependent ATPase activity. This protein’s cellular location dramatically shifts during the cell cycle, indicating its dynamic role.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Human nuclear antigen p68 shares sequence homology with the DEAD box protein family, known for diverse cellular roles.
- These proteins are implicated in fundamental processes such as translation, ribosome assembly, and development.
Purpose of the Study:
- To investigate the enzymatic activity of human nuclear antigen p68.
- To determine the cellular localization dynamics of human nuclear antigen p68 throughout the cell cycle.
Main Methods:
- Immunopurification of human nuclear antigen p68.
- Assay for RNA-dependent ATPase activity.
- Cellular localization studies using microscopy during different cell cycle phases.
Main Results:
- Immunopurified human p68 demonstrated RNA-dependent ATPase activity.
- Human p68 undergoes significant changes in its cellular location during the cell cycle.
Conclusions:
- Human nuclear antigen p68 possesses RNA-dependent ATPase activity, characteristic of helicase superfamily members.
- The dynamic cellular localization of p68 suggests its involvement in cell cycle regulation and other fundamental cellular processes.