Immunological detection of human phosphoglucomutase (PGM 1) subtypes
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Published on: November 8, 2016
D B Whitehouse1, G A Drago, D A Hopkinson
1MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London, U.K.
Abstract:
Anti-phosphoglucomutase (PGM) antibodies have been produced by immunising a sheep with a purified preparation of rabbit skeletal muscle PGM and used to devise an immunological procedure for detecting PGM isozymes after isoelectric focusing. The anti-rabbit PGM antibodies cross react with human PGM and can be used to identify the PGM1 isozymes characteristic of this polymorphism. The patterns revealed by immunodetection are exactly comparable with those obtained by isozyme staining.
