Related Experiment Video
Updated: Apr 23, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
Multidrug-resistant organisms contaminating supply carts of contact isolation patients
Shane Zelencik1, Donna Schora2, Adrienne Fisher1
1Department of Infection Control, NorthShore University HealthSystem, Evanston, IL.
Abstract:
Contamination of supply carts stored within rooms of patients on contact isolation for multidrug-resistant organisms was assessed. Despite the presence of environmentally persistent organisms, very little contamination occurred to these carts or the supplies stored within them. A single isolate containing a multidrug-resistant Acinetobacter baumannii was isolated, representing 1.3% of the 80 swabs collected.
Insights
Supply carts in patient rooms with multidrug-resistant organisms showed minimal contamination. Even with persistent microbes, carts and supplies remained largely clean, indicating effective isolation protocols.
Area of Science:
- Infection Control and Prevention
- Microbiology
- Healthcare Epidemiology
Background:
- Multidrug-resistant organisms (MDROs) pose a significant threat in healthcare settings.
- Contact isolation precautions are standard for patients colonized or infected with MDROs.
- Understanding potential reservoirs for MDRO transmission, such as supply carts, is crucial.
Purpose of the Study:
- To assess the level of contamination on supply carts and their contents.
- To determine if MDROs contaminate carts used by patients under contact isolation.
- To evaluate the effectiveness of current practices in preventing cart contamination.
Main Methods:
- Environmental sampling of supply carts and stored supplies was conducted.
- Swabs were collected from various surfaces of the carts and items.
- Samples were analyzed for the presence of multidrug-resistant organisms.
Main Results:
- Very low levels of contamination were detected on supply carts and stored supplies.
- Only one isolate of multidrug-resistant *Acinetobacter baumannii* was identified.
- This represented 1.3% of the 80 swabs collected, suggesting minimal environmental persistence on carts.
Conclusions:
- Supply carts in contact isolation rooms are unlikely to be significant reservoirs for MDRO transmission.
- Current infection control practices appear effective in preventing widespread cart contamination.
- Further vigilance is warranted, but findings suggest low risk from this specific source.
More Related Videos
09:30Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
07:45Preparation and Maintenance of Bioexclusion IsoPositive Cage Experiment for Human Fecal Transplantation into Germ-Free Mice
Published on: February 28, 2025
Related Concept Videos
Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
Transmission-based Precautions I: Contact, Enteric, and Droplets
Contact Precautions:
Contact precautions are the measures taken to prevent the transmission of infectious agents, especially epidemiologically important microorganisms such as MRSA or influenza, primarily transmitted through direct or indirect contact with an...
Clinical Significance of Antibiotic Resistance
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...
Healthcare Associated Infections II: Preventive Measures
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Mechanism of Antibiotic Resistance in MRSA