Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

51.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
51.3K
Translational Regulation01:29

Translational Regulation

865
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
865
Equipments Used to Measure Body Temperature01:13

Equipments Used to Measure Body Temperature

2.0K
Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
2.0K
Assessing Body Temperature - Oral01:14

Assessing Body Temperature - Oral

2.0K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibody Titres to Strangvac<sup>®</sup> Antigens Correlate with Protection and Duration of Immunity Against Experimental Infection with <i>Streptococcus equi</i> Subspecies <i>equi</i>.

Vaccines·2026
Same author

From urge to action: an integrative neuropsychological model of tic generation in Tourette syndrome.

Frontiers in psychiatry·2026
Same author

Pneumococcal membrane particles promote serotype-independent cellular and humoral immunity and protect against pneumococcal colonization.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Toward understanding the catalytic mechanism for two classes of bacterial phospholipid N-methyltransferases.

The FEBS journal·2026
Same author

Fis suppresses late-stage virulence gene expression in Yersinia pseudotuberculosis at environmental temperatures.

PLoS pathogens·2026
Same author

The Ti Plasmid-Encoded VirJ Functions as a Lysyl-Phosphatidylglycerol Hydrolase in Agrobacterium tumefaciens.

Molecular microbiology·2026

Related Experiment Video

Updated: Apr 23, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.5K

How to find RNA thermometers.

Francesco Righetti1, Franz Narberhaus1

  • 1Microbial Biology, Ruhr University Bochum Bochum, Germany.

Frontiers in Cellular and Infection Microbiology
|October 4, 2014
PubMed
Summary

Bacteria sense host temperature using RNA thermometers (RNATs) in mRNA. Next-generation sequencing now globally reveals these novel temperature-responsive RNA structures.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genomics

Background:

  • Pathogenic bacteria utilize host temperature as a signal for adaptation.
  • Bacteria commonly employ temperature-modulated mRNA structures, known as RNA thermometers (RNATs), to regulate gene expression.
  • RNATs are typically found in the untranslated regions of mRNAs, controlling translation initiation.

Purpose of the Study:

  • To review the historical discovery of RNA thermometers (RNATs).
  • To highlight the application of next-generation sequencing for discovering novel temperature-responsive RNA structures.
  • To provide a global perspective on RNATs in bacterial pathogens.

Main Methods:

  • Literature review of RNAT discovery.
  • Application of high-throughput sequencing technologies (e.g., RNA-Seq).
Keywords:
RNA structureheat shocknext-generation sequencingregulatory RNAvirulence

More Related Videos

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

969
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

23.2K

Related Experiment Videos

Last Updated: Apr 23, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

10.5K
Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

969
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

23.2K
  • Bioinformatic analysis of RNA structures and temperature sensitivity.

    • Main Results:

    • Historically, RNATs were identified individually upstream of specific genes (e.g., heat shock, virulence).
    • Next-generation sequencing enables large-scale identification of previously unknown RNATs.
    • Global analysis reveals a broader prevalence and diversity of temperature-responsive RNA structures.

    Conclusions:

    • RNA thermometers are crucial for bacterial adaptation to host environments.
    • Next-generation sequencing revolutionizes the discovery and characterization of RNATs.
    • Understanding RNATs offers potential targets for antimicrobial strategies.