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Updated: Apr 23, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Colorimetric aptasensor using unmodified gold nanoparticles for homogeneous multiplex detection.

Shucao Niu¹, Zhenzhen Lv¹, Jinchuan Liu¹

¹Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, China; Key Laboratory of Agri-Food Quality and Safety, Ministry of Agriculture, Beijing, China.

|October 4, 2014

Summary

This summary is machine-generated.

Researchers developed a colorimetric multiplex aptasensor using gold nanoparticles (AuNPs) to detect multiple targets simultaneously. This practical assay offers sensitive detection of sulfadimethoxine, kanamycin, and adenosine, maintaining single-target sensitivity.

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Area of Science:

Nanotechnology and Nanosensors
Biochemical Assays and Diagnostics
Molecular Recognition

Background:

Colorimetric aptasensors utilizing unmodified gold nanoparticles (AuNPs) are cost-effective and practical for various targets.
Existing research predominantly focuses on single-target detection, limiting simultaneous analysis capabilities.
A need exists for versatile, multiplexed aptasensor platforms for simultaneous detection of multiple analytes.

Purpose of the Study:

To develop a homogeneous multiplex colorimetric aptasensor capable of simultaneously detecting multiple targets.
To demonstrate the feasibility of using multiple aptamers to stabilize AuNPs for multiplexed detection.
To evaluate the sensitivity and performance of the multiplex aptasensor compared to single-target assays.

Main Methods:

Mixed aptamers for kanamycin (KAN), sulfadimethoxine (SDM), and adenosine (ADE) were adsorbed onto unmodified AuNPs via electrostatic interaction.
Target-induced conformational changes in aptamers altered their stabilizing effect on AuNPs.
AuNP aggregation and subsequent color change (red to purple/blue) were observed in a specific buffer upon target presence.

Main Results:

The developed aptasensor successfully detected the presence of KAN, SDM, and ADE simultaneously.
The multiplex aptasensor maintained sensitivity comparable to individual single-target aptasensors for each analyte.
The assay exhibited a distinct colorimetric response, enabling visual detection of target analytes.

Conclusions:

A homogeneous multiplex colorimetric aptasensor strategy was successfully developed for simultaneous detection of multiple analytes.
This approach offers a simple, cost-effective, and rapid method for screening samples with multiple targets.
The platform is extensible to other aptamers, providing a powerful tool for diverse molecular detection applications.