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Related Concept Videos

Ribozymes02:47

Ribozymes

10.0K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
10.0K
Ribozymes02:47

Ribozymes

2.7K
2.7K
Types of RNA01:20

Types of RNA

13.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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Types of RNA01:23

Types of RNA

61.0K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
61.0K
Riboswitches01:56

Riboswitches

7.9K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
7.9K
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Apr 23, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

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Looking at LncRNAs with the ribozyme toolkit.

Anna Marie Pyle1

  • 1Department of Molecular, Cellular and Developmental Biology and Department of Chemistry, Yale University, New Haven, CT 06520, USA.

Molecular Cell
|October 4, 2014
PubMed
Summary

Understanding RNA structure is crucial for cellular function. New technologies now enable visualization of diverse RNA types, offering mechanistic insights into their roles.

Area of Science:

  • Molecular biology
  • Structural biology
  • Genetics

Background:

  • Large RNA molecules are essential for cellular functions.
  • The molecular structures of many RNA types remain poorly understood.
  • Existing technologies offer limited insights into RNA structure.

Purpose of the Study:

  • To highlight the importance of understanding RNA structures.
  • To introduce novel technologies for RNA visualization.
  • To facilitate mechanistic insights into RNA functions.

Main Methods:

  • Development of robust technologies for ribozyme and riboswitch visualization.
  • Implementation of new approaches for intracellular RNA mapping.
  • Application of these methods to long noncoding RNAs, mRNAs, and viral RNAs.

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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Related Experiment Videos

Last Updated: Apr 23, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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Main Results:

  • Established a foundation for visualizing diverse RNA structures.
  • Enabled detailed examination of RNA molecular architecture.
  • Provided new avenues for mechanistic studies of RNA molecules.

Conclusions:

  • Advancements in RNA visualization technologies are critical.
  • Understanding RNA structure will unlock new mechanistic insights.
  • This work paves the way for future RNA-focused research.