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Related Experiment Videos

An oligonucleotide probe specific for Onchocerca volvulus.

W Harnett1, A E Chambers, A Renz

  • 1Division of Parasitology, National Institute for Medical Research, London, U.K.

Molecular and Biochemical Parasitology
|June 15, 1989
PubMed
Summary
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Researchers developed a species-specific DNA probe for Onchocerca volvulus, the parasite causing river blindness. This new probe, C1A1-2, accurately identifies O. volvulus DNA, aiding in disease diagnosis and control efforts.

Area of Science:

  • Molecular Biology
  • Parasitology
  • Genetics

Background:

  • Onchocerca volvulus is a parasitic nematode responsible for onchocerciasis (river blindness).
  • Accurate identification of O. volvulus is crucial for disease control and epidemiological studies.
  • Existing diagnostic methods may lack specificity or require complex procedures.

Purpose of the Study:

  • To develop a highly specific molecular probe for the detection of Onchocerca volvulus.
  • To identify unique genetic sequences for species-specific parasite identification.

Main Methods:

  • Construction of a genomic DNA library from O. volvulus in a bacteriophage lambda gt10 vector.
  • Differential screening of the library using homologous and heterologous parasite DNA, as well as human DNA.

Related Experiment Videos

  • Subcloning and sequencing of a promising clone (C1A1).
  • Synthesis and hybridization testing of oligonucleotides derived from the unique sequence with various Onchocerca species.

    • Main Results:

    • Isolation of clone C1A1 with strong binding to O. volvulus DNA and no binding to human DNA.
    • Identification of a 60-nucleotide unique region within the C1A1 insert.
    • Development of oligonucleotide C1A1-2, which hybridized strongly (300x greater) to all tested O. volvulus isolates compared to other Onchocerca species.
    • High specificity observed even against closely related species like O. gibsoni and O. ochengi.

    Conclusions:

    • The oligonucleotide C1A1-2 functions as a highly species-specific probe for Onchocerca volvulus.
    • This probe has significant potential for accurate diagnosis and monitoring of onchocerciasis.
    • The developed probe offers a valuable tool for epidemiological research and parasite control strategies.