Bio-electrospraying is a safe technology for delivering human adipose-derived stem cells

Area of Science:

• Biotechnology
• Regenerative Medicine
• Cell Biology

Background:

• Bio-electrospraying (BES) is a novel technique for direct cell deposition.
• Its application in tissue engineering and regenerative medicine is promising.
• The impact of BES on human adipose-derived stem cells (hASCs) is not yet understood.

Purpose of the Study:

• To investigate the effects of bio-electrospraying on the viability, morphology, and multipotency of human adipose-derived stem cells (hASCs).
• To determine optimal BES parameters for preserving hASC function.

Main Methods:

• hASC suspensions were subjected to bio-electrospraying at varying voltages (0 kV, 10 kV, 20 kV).
• Cell morphology was assessed using microscopy.
• Cell viability and proliferation were evaluated using Trypan Blue and CCK-8 assays.
• Multilineage differentiation potential was tested after BES treatment.

Main Results:

• Stable and continuous electrospray of hASCs was achieved at 10 kV and 3 ml/h.
• hASCs electrosprayed at 10 kV exhibited no significant changes in morphology, viability, or proliferation compared to controls.
• Cell viability decreased, and BES became unstable at 20 kV.
• hASCs treated at 10 kV retained their ability to differentiate into chondrogenic, osteogenic, and neurogenic lineages.

Conclusions:

• Bio-electrospraying at 10 kV is a viable method for processing hASCs without compromising their key characteristics.
• BES preserves hASC morphology, viability, and multipotency, making it suitable for regenerative medicine.
• Optimizing BES parameters is critical to avoid adverse effects on cell function.