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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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Analyzing the biosensor signal in flows: studies with glucose optrodes.

K Kivirand1, A Floren2, M Kagan1

  • 1Institute of Chemistry, University of Tartu, Ravila 14a, Tartu 50411, Estonia.

Talanta
|October 5, 2014
PubMed
Summary

A new model enables rapid determination of biosensor signal parameters for quick calibration. This method enhances biosensor stability and extends the effective lifetime of enzymatic bio-optrodes.

Keywords:
Dual-optrode biosensorFlow regimeGlucoseModelingPre-steady state calibration

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Enzymatic bio-optrodes are crucial for biosensing applications.
  • Accurate calibration and long-term stability are key challenges in biosensor development.

Purpose of the Study:

  • To develop a reliable method for rapid determination of biosensor signal parameters.
  • To establish a model for quick biosensor calibration.
  • To design a biosensor system with reduced enzyme inactivation and prolonged effective lifetime.

Main Methods:

  • Studied enzymatic bio-optrode responses in a flow regime.
  • Developed a dual-optrode glucose biosensor (glucose and oxygen optrodes).
  • Proposed an original model for signal parameter determination from the initial transient phase.

Main Results:

  • Biosensor signal parameters were independent of probe flow speed.
  • Parameters could be determined from the initial transient phase signal for rapid analysis.
  • The model design reduced the impact of enzyme inactivation on system stability.

Conclusions:

  • A novel model allows for rapid biosensor calibration.
  • The developed biosensor system exhibits enhanced stability and extended operational lifetime.
  • This approach offers a valuable tool for efficient and reliable biosensing.