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Related Experiment Video

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A profluorescent ratiometric probe for intracellular pH imaging.

Qingqing Zhang1, Ming Zhou2

  • 1Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu 215123, PR China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, PR China; Institute of Chemistry, Chinese Academy of Sciences, 2 Zhongguancun North First Street, Beijing 100190, PR China.

Talanta
|October 5, 2014
PubMed
Summary

Researchers developed a novel ratiometric pH probe using iridium(III) complex for accurate cellular pH monitoring. This probe shows linear responses within the physiological range, aiding in biological research.

Keywords:
FluoresceinIntracellular pHIridium(III) complexNear-infraredProfluorescentRatiometric

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Area of Science:

  • Chemical Biology
  • Materials Science
  • Analytical Chemistry

Background:

  • Accurate monitoring of intracellular pH is crucial for understanding cellular processes.
  • Developing sensitive and specific pH probes is essential for biological and medical research.

Purpose of the Study:

  • To synthesize and characterize a novel ratiometric pH probe.
  • To evaluate the probe's performance in detecting pH variations in a physiological range within living cells.

Main Methods:

  • Synthesis of a ratiometric pH probe combining a fluorescein moiety and a cyclometalated iridium(III) complex.
  • Assessment of the probe's cell permeability and ratiometric response to pH changes.
  • In vitro testing using HeLa cell assays.

Main Results:

  • The synthesized probe exhibited good cell permeability.
  • A linear ratiometric response to pH variations was observed in the physiological range.
  • The probe successfully detected pH changes in HeLa cells.

Conclusions:

  • The developed iridium(III)-based probe is a promising tool for ratiometric pH sensing in biological systems.
  • Its ability to function within the physiological pH range makes it suitable for cellular studies.
  • This probe offers potential for advancing research in cell biology and disease diagnostics.