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Updated: Apr 23, 2026
In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography
Published on: February 16, 2016
Closed-chest small animal model to study myocardial infarction in an MRI environment in real time
Darach O h-Ici1, Sarah Jeuthe, Thore Dietrich
1Unit for Cardiovascular Imaging, Department of Congenital Heart Disease and Pediatric Cardiology, Deutsches Herzzentrum Berlin, Augustenburger Platz 1, 13353, Berlin, Germany, darachohici@gmail.com.
Abstract:
Current models for real time study of the effects of myocardial ischemia/reperfusion have major limitations and confounders. Confounders include the surgical stresses of a thoracotomy and abnormal physiology of an open chest. The need to reposition the animal interferes with the study of the early changes associated with ischemia. Direct comparison of pre-ischemia and post-ischemia images is then difficult. We developed a novel "closed chest" model of ischemia/reperfusion to overcome these issues. Following thoracotomy, we sutured a balloon occluder to the left coronary artery of male Sprague-Dawley rats. We used both visual inspection and ECG to assess for successful occlusion and reperfusion of the coronary artery at the time of operation by brief inflation and deflation of the balloon. The tubing was then placed under the skin and the incision closed. Following a recovery period (5-10 days), the animals underwent MRI. We performed baseline assessment of left ventricle function, and repeated LV measurement during a 15-min coronary occlusion and again during a 60-min reperfusion period following reopening of the coronary artery. The occluder was successfully placed in 40 of 44 animals. Four developed intraoperative complications; two large myocardial infarction, two terminal bleeding. Six died in the week following surgery, [four sudden deaths (presumed arrhythmic), one anterior infarction, one sepsis]. Cine-MRI demonstrated localised hypokinesia in 31 of the remaining 34 animals. LV ejection fraction (EF) was reduced from 63 ± 7 % at baseline, to 49 ± 9 % during coronary occlusion. LV EF recovered to 61 ± 2 %. The area at risk on staining of the heart was 41.9 ± 15.8 %. This method allows the effects of ischemia/reperfusion to be studied before, during, and after coronary occlusion. Ischemia can be caused while the animal is in the MRI. This new and clinically relevant small animal model is a valuable tool to study the effects of single or repeated coronary occlusion/reperfusion in real-time.
Insights
This study introduces a new closed-chest model for studying myocardial ischemia/reperfusion in rats. This innovative technique allows real-time MRI assessment of heart function before, during, and after coronary occlusion, overcoming limitations of previous methods.
Area of Science:
- Cardiovascular Research
- Animal Models
- Medical Imaging
Background:
- Current myocardial ischemia/reperfusion models have significant limitations.
- Surgical stress and open-chest procedures confound real-time studies.
- Difficulty in comparing pre- and post-ischemia imaging hinders early change analysis.
Purpose of the Study:
- To develop and validate a novel closed-chest model for studying myocardial ischemia/reperfusion.
- To enable real-time assessment of cardiac function using MRI during ischemia and reperfusion.
- To overcome limitations of existing animal models for cardiovascular research.
Main Methods:
- Developed a closed-chest model in Sprague-Dawley rats using a balloon occluder on the left coronary artery.
- Assessed occlusion and reperfusion via visual inspection and ECG.
- Performed serial MRI to measure left ventricle function (ejection fraction) at baseline, during occlusion, and during reperfusion.
Main Results:
- Successful occlusion and reperfusion model achieved in 40 of 44 rats.
- Cine-MRI showed localized hypokinesia in 31 animals.
- Left ventricle ejection fraction decreased during occlusion (63% to 49%) and recovered post-reperfusion (to 61%).
- Area at risk was 41.9%.
Conclusions:
- The novel closed-chest model allows real-time, in-situ study of ischemia/reperfusion effects.
- This clinically relevant small animal model is valuable for investigating coronary occlusion/reperfusion.
- Enables pre-ischemia, during-ischemia, and post-reperfusion assessments within MRI.
