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Tissue distribution and ontogeny of multidrug resistance protein 2, a phosphatidylcholine translocator, in rats

Qiuyang Zhang1, Wei Yang1, Hanlin Song1

  • 1Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Mailbox 210, #24 Tongjiaxiang, Nanjing, 210009, Jiangsu, People's Republic of China.

European Journal of Drug Metabolism and Pharmacokinetics
|October 7, 2014
PubMed

Insights

Multidrug resistance protein 2 (Mdr2) is crucial for phospholipid transport. This study reveals its distribution in rat tissues, age-dependent development, and sex differences, highlighting post-transcriptional regulation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Multidrug resistance protein 2 (Mdr2) is essential for phosphatidylcholine translocation.
  • Understanding Mdr2 regulation in Sprague-Dawley rats is vital for pre-clinical research.

Purpose of the Study:

  • To investigate the tissue distribution of Mdr2 in rats.
  • To determine gender-specific expression patterns of Mdr2.
  • To elucidate the ontogeny of Mdr2 at both gene and protein levels.

Main Methods:

  • Quantitative analysis of Mdr2 gene and protein expression across various rat tissues.
  • Assessment of Mdr2 expression in relation to gender and age.

Main Results:

  • Mdr2 exhibits high expression in the liver, moderate levels in the brain and testes, and lower levels in the gastrointestinal tract.
  • Significant gender divergence in Mdr2 mRNA expression was observed, predominantly in males in liver and reproductive organs.
  • The developmental pattern of Mdr2 protein did not perfectly mirror mRNA levels, suggesting post-transcriptional regulation.

Conclusions:

  • Rat Mdr2 is distributed in the brain, testes, and intestine, in addition to the liver.
  • Mdr2 ontogeny follows an age-dependent pattern, influenced by post-transcriptional regulatory mechanisms.
Keywords:
DistributionDrug-induced liver injuryMultidrug resistance protein 2Multidrug resistance protein 3OntogenyPost-transcriptional regulation

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