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Updated: Apr 23, 2026
Isolation and Culture Expansion of Tumor-specific Endothelial Cells
Published on: October 14, 2015
Suprabasin as a novel tumor endothelial cell marker
Mohammad T Alam1, Hiroko Nagao-Kitamoto, Noritaka Ohga
1Vascular Biology, Frontier Research Unit, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan; Department of Oral Pathology and Biology, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Suprabasin (SBSN) is upregulated in tumor endothelial cells (TEC), promoting their migration and tube formation. This suggests SBSN plays a role in tumor angiogenesis and could serve as a novel TEC marker.
Area of Science:
- Oncology
- Cell Biology
- Biochemistry
Background:
- Stromal cells, including tumor endothelial cells (TEC), are increasingly recognized for their role in tumor progression.
- Previous research indicated distinct characteristics between TEC and normal endothelial cells (NEC).
- Gene profiling revealed suprabasin (SBSN) upregulation in TEC compared to NEC, but its function remained unclear.
Purpose of the Study:
- To investigate the role of suprabasin (SBSN) in tumor endothelial cells (TEC).
- To determine if SBSN influences the angiogenic potential of TEC.
- To explore SBSN as a potential novel TEC marker.
Main Methods:
- Gene expression analysis comparing SBSN levels in isolated human and mouse TEC versus NEC.
- Functional assays including cell migration and tube formation assays following SBSN knockdown in TEC.
- Investigation of downstream signaling pathways, specifically the AKT pathway.
Main Results:
- SBSN expression was significantly higher in TEC compared to NEC.
- Knockdown of SBSN impaired the migratory and tube formation capabilities of TEC.
- The AKT pathway was identified as a downstream target of SBSN signaling.
Conclusions:
- Suprabasin (SBSN) is implicated in the angiogenic properties of tumor endothelial cells (TEC).
- SBSN influences TEC migration and tube formation, potentially via the AKT pathway.
- SBSN may represent a novel biomarker for TEC and a therapeutic target in cancer.
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