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Updated: Apr 23, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
Published on: February 7, 2025
Advances in mass spectrometry driven O-glycoproteomics
Steven B Levery1, Catharina Steentoft1, Adnan Halim1
1Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark.
Proteome-wide analysis of O-glycosylation sites is now possible, revealing new biological functions. Further advancements are needed for detailed glycan structure analysis in both N- and O-glycoproteomics.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- Mass spectrometry is crucial for global protein analysis in biomedical research.
- Glycoprotein analysis, particularly O-glycans, presents significant challenges.
- While N-glycoprotein analysis is established, O-glycoproteomics is rapidly advancing.
Purpose of the Study:
- To review challenges in O-glycan analysis.
- To discuss novel O-glycoproteomics strategies for O-GalNAc and O-Man glycoproteomes.
Main Methods:
- Focus on O-glycoproteomics strategies.
- Analysis of O-GalNAc and O-Man glycoproteomes.
Main Results:
- Proteome-wide O-glycosylation site analysis is now achievable.
- New biological functions of O-glycosylation have been identified.
- Understanding of O-glycan positioning on proteins has improved.
Conclusions:
- Multiple strategies exist for proteome-wide O-glycosylation site analysis, facilitating functional studies.
- Further development is required for comprehensive analysis of glycan structures at individual sites for both N- and O-glycoproteomics.
- Advances in O-glycoproteomics enhance understanding of O-glycosylation's biological roles and protein localization.
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