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Anion atpases from rainbow trout gills showing high and low anion affinity
1Department of Biological Sciences, The University, Dundee, Scotland.
Abstract:
1. Chloride-bicarbonate ATPase shows two affinity constants for the bicarbonate ion; a low affinity constant (Km=12.5mEq/1) and a high affinity constant (Km=0.17mEq/1), suggesting that two separate enzymes may exist. 2. In the presence of 5mEq/1 Cl- the low affinity Km is increased to 2.5mEq/1. 3. Chloride alone does not activate the enzyme, but in the presence of 4mEq/1 HCO3- the Km value is 5mEq/1.