Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.1K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.1K
Forced Transdifferentiation01:28

Forced Transdifferentiation

1.5K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
1.5K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.0K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.0K
Metastasis02:30

Metastasis

5.4K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.4K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Seasonal variation of reproductive performance, foetal development and progesterone concentrations of sheep in the subtropics.

Reproduction in domestic animals = Zuchthygiene·2008
Same author

Validation of a soccer skill test for use with females.

International journal of sports medicine·2008
Same author

Assessment of the need to coat particle collection cups of the NGI to mitigate droplet bounce when evaluating nebuliser-produced droplets.

Pharmeuropa scientific notes·2008
Same author

Cooling the NGI - an approach to size a nebulised aerosol more accurately.

Pharmeuropa scientific notes·2008
Same author

Multiplex PCR for differential diagnosis of emerging typhoidal pathogens directly from blood samples.

Epidemiology and infection·2008
Same author

Gelrite microgels for sustained oral drug delivery-formulation and evaluation.

Current drug delivery·2008

Related Experiment Video

Updated: Apr 23, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.0K

Metabolic reprogramming during TGFβ1-induced epithelial-to-mesenchymal transition.

L Jiang1, L Xiao2, H Sugiura3

  • 1Children's Medical Center Research Institute, Dallas, TX, USA.

Oncogene
|October 7, 2014
PubMed
Summary

Cancer cells undergoing epithelial-to-mesenchymal transition (EMT) suppress fat production (lipogenesis) and increase energy production. This metabolic shift, driven by Snail1, promotes cancer metastasis and patient death.

More Related Videos

Induction and Analysis of Epithelial to Mesenchymal Transition
10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

38.0K
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
06:02

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

12.0K

Related Experiment Videos

Last Updated: Apr 23, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

13.0K
Induction and Analysis of Epithelial to Mesenchymal Transition
10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

38.0K
Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
06:02

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

12.0K

Area of Science:

  • Cancer Biology
  • Metabolic Reprogramming
  • Metastasis Research

Background:

  • Metastatic progression is the primary cause of cancer mortality.
  • Cancer cells alter metabolism, favoring glycolysis and biosynthesis, but regulatory changes during metastasis are unclear.
  • Transforming growth factor beta 1 (TGFβ1) induces epithelial-to-mesenchymal transition (EMT), a key process in metastasis.

Purpose of the Study:

  • To define metabolic changes and regulatory gene expression during metastatic progression.
  • To investigate the role of lipogenesis suppression in TGFβ1-induced EMT and metastasis.

Main Methods:

  • Analyzed enzyme expression during TGFβ1-induced EMT.
  • Investigated the effect of Snail1 overexpression on lipogenic regulators (ChREBP, FASN).
  • Utilized stable FASN knockdown in vitro and in vivo models to assess EMT, migration, extravasation, and metastasis.

Main Results:

  • TGFβ1-induced EMT reduced enzymes for glucose-to-fatty acid conversion and increased respiration.
  • Snail1 suppressed ChREBP and FASN expression.
  • FASN knockdown induced EMT, enhanced migration and extravasation in vitro, and increased lung metastasis and mortality in vivo.

Conclusions:

  • A metabolic shift suppressing lipogenesis and favoring energy production is crucial for TGFβ1-induced EMT.
  • This metabolic transition is an essential driver of cancer metastasis.
  • Targeting lipogenesis pathways may offer novel therapeutic strategies for metastatic cancer.