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3D printed metal columns for capillary liquid chromatography.

S Sandron1, B Heery, V Gupta

  • 1Australian Centre for Research on Separation Sciences (ACROSS), and ARC Centre of Excellence for Electromaterials Science, School of Physical Sciences, University of Tasmania, Australia. Brett.Paull@utas.edu.au.

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Summary

3D printed coiled capillary chromatography columns were fabricated from stainless steel and titanium alloys. These novel columns, packed with particles or monoliths, show promise for portable analytical devices.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Chromatography is a crucial analytical technique for separating complex mixtures.
  • Traditional chromatography columns can be bulky and expensive, limiting portability.
  • Additive manufacturing offers new possibilities for fabricating customized chromatographic devices.

Purpose of the Study:

  • To develop and evaluate 3D printed coiled planar capillary chromatography columns.
  • To assess the suitability of stainless steel and titanium alloys for column fabrication.
  • To explore packing methods using reversed-phase particles and in situ monolith preparation.

Main Methods:

  • Coiled planar columns (0.9 mm I.D. × 60 cm L) were 3D printed using 316L stainless steel and Ti-6Al-4V titanium alloys.
  • Columns were slurry packed with octadecylsilica (ODS) particles or filled with a methacrylate-based monolith.
  • Printed columns were integrated with Peltier thermoelectric heater/cooler modules for temperature control.

Main Results:

  • Successful fabrication of 3D printed coiled capillary chromatography columns in both stainless steel and titanium.
  • Demonstrated feasibility of packing columns with ODS particles and preparing in situ monoliths.
  • Preliminary integration with thermoelectric modules indicates potential for temperature-controlled portable systems.

Conclusions:

  • 3D printing provides a viable route for manufacturing advanced coiled planar chromatography columns.
  • The use of metal alloys offers robustness and potential for miniaturization.
  • These 3D printed columns represent a promising advancement for the development of portable chromatographic instruments.