Related Experiment Video
Updated: Apr 23, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
Published on: December 28, 2017
Changes in genotype and fluconazole susceptibility of isolates from patients with Candida glabrata in Tunisia
Salma Abbes1, Imen Amouri1, Hayet Sellami1
1Laboratoire de biologie moléculaire, parasitaire et fongique, Faculté de médecine, Université de Sfax, Tunisie.
Unlabelled:
Candida glabrata has emerged as an opportunistic pathogen of considerable importance in invasive and superficial infections.
Aims:
To analyze the development of fluconazole resistance in patients under treatment through epidemiological survey in our hospital.
Patients And Methods:
Twenty two patients (89 clinical strains) were collected. Molecular typing of isolates was performed by polymorphic markers. Analysis of gene expression was realized by reverse transcriptase-real time polymerase chain reactions (RT-qPCR).
Results:
Genetic analysis showed that 63% persists with apparently unchanged strains (n=14). Among them, four showed fluconazole resistance development. A strain replacement was observed in 6 patients and two patients selected more resistant isolates during the course of treatment. An analysis of Candida glabrata cerebellar degeneration-related protein 1 (CgCDR1), Candida glabrata cerebellar degeneration-related protein 2 (CgCDR2) and Candida glabrata sterol 14 alpha-demetylase Erg 11 (CgERG11) expression revealed an over-expression in 10 resistant isolates.
Conclusion:
This study demonstrated that C. glabrata strain undergo frequent changes in vivo. The increase in CgCDR1 and CgCDR2 expression was the most mechanism associated with fluconazole resistance.
More Related Videos
09:06Author Spotlight: Photodynamic Therapy as a Novel Approach to Induce Petite Colonies in Drug-Resistant Candida for Antifungal Research
Published on: March 29, 2024
08:48Bio-energetics Investigation of Candida albicans Using Real-time Extracellular Flux Analysis
Published on: March 19, 2019
Related Concept Videos
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Antifungal Agents
Inhibitors of Bacterial DNA Synthesis