Human skeletal muscle biopsy procedures using the modified Bergström technique

Area of Science:

• Sports Medicine
• Human Physiology
• Biotechnology

Background:

• Percutaneous biopsy is a crucial method for obtaining skeletal muscle tissue samples.
• Assessing muscle tissue is vital for research and clinical applications.
• Existing biopsy techniques may have limitations in efficiency or invasiveness.

Purpose of the Study:

• To describe and demonstrate a modified Bergström needle technique for percutaneous skeletal muscle biopsy.
• To highlight the safety and efficacy of this specific biopsy method.
• To provide a procedural overview for obtaining skeletal muscle samples from the vastus lateralis.

Main Methods:

• Utilizes a modified Bergström needle with an outer cannula and inner trocar.
• Involves local anesthesia and aseptic techniques for needle insertion.
• Employs suction and a cutting mechanism to obtain muscle tissue samples (100-200 mg).

Main Results:

• The technique is safe and highly effective for skeletal muscle sampling.
• Multiple cuts can yield sufficient tissue for analysis.
• Subjects can resume daily activities quickly post-procedure.

Conclusions:

• The modified Bergström needle technique offers a reliable method for percutaneous skeletal muscle biopsy.
• This technique facilitates timely tissue acquisition relative to exercise or interventions.
• Rapid recovery allows for prompt return to physical activity, with precautions against heavy resistance exercise.