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Accessory cell function of Th2 clones.

B D Evavold1, J Quintans

  • 1Department of Pathology, University of Chicago, IL 60637.

Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 1989
PubMed
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T helper 2 (Th2) cells, but not T helper 2 (Th2) cells, act as accessory cells. They activate splenic T cells to proliferate when combined with mitogens, independent of MHC restriction.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T helper (Th) cells play crucial roles in adaptive immunity.
  • Accessory cells (AC) are essential for T cell activation and proliferation.
  • Understanding the specific functions of Th cell subsets as AC is vital for immune response modulation.

Purpose of the Study:

  • To investigate the capacity of T helper clones to function as accessory cells.
  • To determine the role of different T helper cell subsets in activating naive splenic T cells.
  • To elucidate the mechanisms underlying T cell activation by accessory Th cells in the presence of mitogens.

Main Methods:

  • Costimulatory assays using cloned T helper cells and freshly isolated splenic T cells.
  • Utilizing various T cell mitogens, including Concanavalin A (Con A) and monoclonal antibodies (mAbs) against CD3, TCR, and Thy-1.

Related Experiment Videos

  • Analyzing dose-response curves and assessing the involvement of MHC restriction.
  • Investigating the proliferation of CD4+ and CD8+ splenic T cells using specific mAbs.

    • Main Results:

    • T helper cells secreting Interleukin-4 (IL-4), identified as Th2 clones, effectively functioned as accessory cells.
    • Th2 cells induced splenic T cell proliferation in the presence of mitogens, independent of MHC restriction.
    • The activation signal involved a single stimulator cell and was not dependent on CD4 or CD8 expression on the responding T cells.

    Conclusions:

    • Cloned Th2 cells possess accessory cell function.
    • Th2 cells, in conjunction with mitogens, can induce proliferation of naive splenic T cells.
    • This Th2-mediated activation is MHC-independent and involves a single stimulator cell interaction.