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Related Experiment Videos

Laminin promotes mast cell attachment.

H L Thompson1, P D Burbelo, B Segui-Real

  • 1Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

Journal of Immunology (Baltimore, Md. : 1950)
|October 1, 1989
PubMed
Summary
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Mast cells have functional laminin receptors, explaining their location near blood vessels and accumulation during inflammation. Interleukin-3 (IL-3) enhances this interaction and receptor expression.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Tissue mast cells are frequently found near endothelial cell basement membranes.
  • Mast cell accumulation at inflammatory sites is a known phenomenon.
  • The underlying mechanisms for mast cell tissue distribution remain unclear.

Purpose of the Study:

  • To investigate the interaction between mast cells and laminin.
  • To determine if mast cells express functional laminin receptors.
  • To elucidate the role of laminin receptors in mast cell tissue localization.

Main Methods:

  • Utilized murine mast cell line (PT18) and bone marrow-derived mast cells.
  • Assessed mast cell adhesion, spreading, and histamine granule redistribution on laminin substrata.

Related Experiment Videos

  • Investigated the effect of Interleukin-3 (IL-3) on mast cell adherence.
  • Employed antibody inhibition assays for laminin and its receptors.
  • Analyzed laminin receptor mRNA expression using Northern blot.

    • Main Results:

    • Mast cells (PT18 and bone marrow-derived) possess functional laminin receptors.
    • Mast cells demonstrated adhesion, spreading, and granule redistribution on laminin.
    • Adherence was enhanced by IL-3 and inhibited by specific antibodies.
    • High expression of 32-kDa laminin receptor mRNA was observed in PT18 cells.
    • IL-3 rapidly induced laminin receptor mRNA expression in bone marrow-derived mast cells.

    Conclusions:

    • Mast cells express functional laminin receptors.
    • These receptors likely mediate mast cell adhesion to the basement membrane.
    • IL-3 influences mast cell adherence and laminin receptor expression.
    • Findings provide a molecular basis for mast cell tissue distribution and inflammatory site accumulation.