Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

4.7K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.7K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.1K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.1K
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

12.3K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
12.3K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

16.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.5K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.0K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

9.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FOXJ1 transcriptional targets in human airway cells and impaired multiciliogenesis in FOXJ1-associated primary ciliary dyskinesia.

American journal of respiratory cell and molecular biology·2026
Same author

Nicotinamide metabolism is essential for Hepatitis C Virus replication and the production of infectious Lipo-Viro-Particles.

PLoS pathogens·2026
Same author

Entry, replication and innate immunity evasion of BANAL-236, a SARS-CoV-2-related bat virus, in Rhinolophus and human cells.

PLoS pathogens·2026
Same author

The Crimean-Congo haemorrhagic fever virus hijacks the liver lipid metabolic pathway for virion production.

Emerging microbes & infections·2026
Same author

Platelet dense granule defect: experience in the French population.

Research and practice in thrombosis and haemostasis·2026
Same author

Enigma Portal Case: Exceptional Urinary Crystals.

Cytopathology : official journal of the British Society for Clinical Cytology·2025

Related Experiment Videos

Virus-induced double-membrane vesicles.

Emmanuelle Blanchard1, Philippe Roingeard

  • 1INSERM U966, Université François Rabelais and CHRU de Tours, Tours, Cedex 37032, France.

Cellular Microbiology
|October 8, 2014
PubMed
Summary

Many RNA viruses create double-membrane vesicles (DMVs) for replication. This review explores DMV biogenesis, structure, and their potential link to autophagy, crucial for viral life cycles.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Cytoplasmic replication of many viruses involves specialized subcellular structures.
  • Positive-strand RNA viruses induce membrane rearrangements, forming double-membrane vesicles (DMVs).
  • DMVs share similarities with autophagosomes, and associated viruses often promote autophagy.

Purpose of the Study:

  • To review recent findings on the biogenesis, architecture, and function of DMVs.
  • To discuss the potential connection between DMVs and autophagy in viral replication.
  • To explore the role of DMVs in the life cycle of diverse viral families.

Main Methods:

  • Literature review of recent studies on viral-induced membrane rearrangements.
  • Analysis of research on double-membrane vesicle formation and characteristics.

Related Experiment Videos

  • Synthesis of data linking viral replication, DMVs, and autophagy pathways.

    • Main Results:

    • Viruses induce complex membrane rearrangements, including DMVs, for efficient replication.
    • DMVs are crucial organelles for viral genome replication and transcription.
    • Evidence suggests a functional link between DMVs and the host cell's autophagic machinery.

    Conclusions:

    • DMVs are essential virus-induced organelles that facilitate viral replication.
    • The relationship between DMVs and autophagy warrants further investigation.
    • Understanding DMV biogenesis and function is key to developing antiviral strategies.